goat anti-human type iii collagen  (SouthernBiotech)

Journal: bioRxiv

Article Title: TLR5 drives metabolic dysfunction-associated steatohepatitis through lipid- and flagellin-induced hepatocyte injury signalling

doi: 10.64898/2026.02.05.703969

Figure Lengend Snippet: Schematic representation of precision-cut liver slices (PCLS) setup, created in Biorender. PCLS were cultured for 16 hours in the presence or absence of 1µM TH1020 (TLR5 inhibitor) and treated with bovine serum albumin (BSA) or oleic acid and palmitic acid (OA PA) for 24 hours (n=3). Representative immunohistochemistry images H&E and Collagen III quantification (% area) of PCLS shown. Scale bar 100µM and 50µM. Error bars represent mean ± standard deviation; P values determined by t test or ANOVA with post-test multiple comparisons. ANOVA, analysis of variance. Only statistically significant comparisons (p<0.05) are highlighted.

Article Snippet: FFPE tissue sections were immunostained with rabbit polyclonal antibody directed against human Collagen Type III (rabbit IgG, ref: 22734-1-A, Proteintech, 1/2000) using the fully automated BOND Max system from Leica Microsystems and a BOND Polymer Refine Detection kit (#DS9800).

Techniques: Cell Culture, Immunohistochemistry, Standard Deviation

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Matrisome analysis of NSCLC unveils clinically-important cancer-associated extracellular matrix changes.

doi: 10.1016/j.bbadis.2025.167709

Figure Lengend Snippet: Fig. 2. Immunofluorescence expression of collagens in the extracellular matrix in lung adenocarcinoma and lung squamous cell carcinoma. The panel shows the negative control, collagen I, collagen III, collagen IV and collagen V. Also as presented the respectively box-plots show the differences of expression between the histological subtypes. The red square in the graphs represents the average expression of the marker for each of the groups. Magnification: 400×. Scale: 100 μm.

Article Snippet: For IF assay, TMA sections were incubated overnight at 4 ◦C with antibodies at a standardized concentration against anti-human collagen type I (600–401–103-0.5; 1:700, Rockland Immunochemicals Inc., Gilbertsville, PA, USA), anti-human collagen type III (600–401–105-0.5; 1:200, Rockland Immunochemicals Inc., Gilbertsville, PA, USA), antihuman collagen type IV (NB120–6586; 1:100, Novus Biologicals, Centennial, CO, USA), and anti-human collagen type V (600–401–107- 0.1; 1:1000, Rockland Immunochemicals Inc., Gilbertsville, PA, USA).

Techniques: Immunofluorescence, Expressing, Negative Control, Marker

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Matrisome analysis of NSCLC unveils clinically-important cancer-associated extracellular matrix changes.

doi: 10.1016/j.bbadis.2025.167709

Figure Lengend Snippet: Fig. 5. Box-plots associate clinicopathological characteristics and extracellular matrix proteins expression in lung squamous cell carcinoma. The elastin expression is associated with N stage (A); chondroitin sulfate expression is associated with age (B); collagen III expression is associated with relapse and/or metastasis (C). The red square in the graphs represents the average expression of the marker for each of the groups. The Kruskal-Wallis’s test was used for median comparison, followed by the Bonferroni Pos-Hoc test. *P < 0.05.

Article Snippet: For IF assay, TMA sections were incubated overnight at 4 ◦C with antibodies at a standardized concentration against anti-human collagen type I (600–401–103-0.5; 1:700, Rockland Immunochemicals Inc., Gilbertsville, PA, USA), anti-human collagen type III (600–401–105-0.5; 1:200, Rockland Immunochemicals Inc., Gilbertsville, PA, USA), antihuman collagen type IV (NB120–6586; 1:100, Novus Biologicals, Centennial, CO, USA), and anti-human collagen type V (600–401–107- 0.1; 1:1000, Rockland Immunochemicals Inc., Gilbertsville, PA, USA).

Techniques: Expressing, Marker, Comparison